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This biocrystalline substance is difficult to degrade and often accumulates in phagocytes. The macrophage response to hemozoin relates to the severity of the disease and the potential for malaria-related disease complications. In this study we have used Raman spectroscopy as a label-free method to investigate the biochemical changes occurring in macrophages during the first few hours of hemozoin uptake.

We found a number of distinct spectral groups, spectrally or spatially related to the presence of the hemozoin inside the cell. Intracellular hemozoin was spectrally identical to extracellular hemozoin, regardless of the location in the cell. A small proportion of hemozoin was found to White pages flushing Frederikshavn associated with lipid-based components, consistent with the uptake of hemozoin into vesicles such as phagosomes and lysosomes.

The spatial distribution of the hemozoin was observed to be inhomogeneous, and its presence largely excluded that of proteins and lipids, demonstrating that cells were not able to break down the biocrystals on the time scales studied.

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These results show that Raman imaging can be used to answer some of the open questions regarding the role Hobo hemozoin in the immune response.

How different combinations of hemozoin and other molecules are treated by macrophages, whether ppages can be broken down by the cell, and more importantly, which co-factors or products are involved in the subsequent Don naestved gay reaction are the expected issues to be elucidated by this technique. The article was received on 15 Ekiaccepted on 17 Oca and first published on 19 Oca Gay gogo bars Danmark Vejle If you are not the author of this article and you wish to reproduce material from it in a third party non-RSC publication you must formally request permission using Copyright Clearance Center.

Go to our Instructions for using Copyright Clearance Center page for details. Authors contributing to RSC publications journal articles, books or book chapters do not need to formally request permission to reproduce material contained in pags article provided that the correct acknowledgement is given with the reproduced material.

If the material has been adapted instead of reproduced from the original RSC publication "Reproduced from" can be substituted with flusihng from". In all cases the Ref.

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XX is the XXth reference in the list of references. If you are the author of this article you do not need to formally request permission to reproduce figures, diagrams. If you are the author of this article you still need to obtain Trans dating Danmark to reproduce the whole article in a third party publication with the exception of reproduction of the whole article in a thesis or dissertation. Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

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